The Phylogeny of Bornean Swamp Buffalo (Bubalus bubalis) Analysis Based on D-loop Mitochondrial DNA Sequence Variation

Swamp buffalo ( Bubalus bubalis ) is one of the Indonesian germplasm that adapts well to Borneo Island. This study aimed determine genetic diversity, phylogenetic tree, and phylogeographic structure Bornean swamp based on control region (D-loop) mitochondrial DNA sequences. A total 120 animals were collected from three populations in Indonesia, i.e., North, East, South Kalimantan Provinces. Along 1140 bp D-loop mtDNA gene buffaloes was amplified with a design primer F: 5'-CAA CAC CCA AAG CTG TT-3’ R: 5'-CGC TCC TCT TAG CGT TG-3’. Therefore, forward reverse sequencing performed visualize full length sequence (1140 bp). Results showed 47 haplotypes detected animal study, haplotype nucleotide diversities 0.936 0.005, respectively. Consequently, two haplogroups observed Haplogroup (North) B (East South). Based analysis, comparable filing China structure. According analysis molecular (AMOVA), geographical component contributed over 56.44% variations. In conclusion, it discovered East identical.